Description
These tracks show the RNA-Seq read coverage for different developmental stages of
D. ananassae. The RNA-Seq reads were mapped against the
D. ananassae (DanaRefSeq2) genome assembly
using HISAT2. RNA-Seq read alignments from
multiple replicates were combined together, and the read coverage was normalized by a wigsum of
100,000,000 (1 million 100bp reads). The read coverage tracks were produced by the bam2wig.py
program in RSeQC.
Accession Numbers for RNA-Seq Data
The RNA-Seq data were obtained from NCBI using the following accession numbers:
Adult Females and Adult Males RNA-Seq Data
Embryos RNA-Seq Data
References
Chen ZX, et al. Comparative validation of
the D. melanogaster modENCODE transcriptome annotation. Genome Res. 2014
Jul;24(7):1209-23.
Kumar N, Creasy T, Sun Y, Flowers M, Tallon LJ, Dunning Hotopp JC. Efficient subtraction of
insect rRNA prior to transcriptome analysis of Wolbachia-Drosophila lateral
gene transfer. BMC Res Notes. 2012 May 14;5:230.
Kim D, Langmead B, Salzberg SL. HISAT: a
fast spliced aligner with low memory requirements. Nat Methods. 2015 Apr;12(4):357-60.
Wang L, Wang S, Li W. RSeQC: quality
control of RNA-Seq experiments. Bioinformatics. 2012 Aug 15;28(16):2184-5.
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