SQANTI3 QC Transcripts Track Settings

Iso-Seq Transcripts Classified by SQANTI3 QC (All PacBio Iso-Seq Tracks)

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Classification based on expression level of other isoforms of the same gene	TRUE if one or more splice junctions could be a RT switching artifact	Structural category based on the best matching reference transcript	Additional splicing categorization

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Data last updated at UCSC: 2022-10-12 22:25:11

Description

Unique isoforms produced by IsoSeq v3 were analyzed by the SQANTI3 Quality Control (QC) workflow. This track shows the collection of unfiltered transcripts produced by SQANTI3 QC. See the Glossary of classification file columns section of the "Understanding the output of SQANTI3 QC" wiki page for an overview of the classification of each transcript.

The color of each feature corresponds to the structural category of the transcript:

Color	Structural Category	Description
	full-splice_match	FSM
	incomplete-splice_match	ISM
	novel_in_catalog	NIC
	novel_not_in_catalog	NNC
	genic	Genic Genomic
	antisense	Antisense
	fusion	Fusion
	intergenic	Intergenic
	genic_intron	Genic Intron

The PacBio Iso-Seq data were produced by Chinmay P. Rele from the Reed Lab at The University of Alabama.

References

Tardaguila M, et al. SQANTI: extensive characterization of long-read transcript sequences for quality control in full-length transcriptome identification and quantification. Genome Res. 2018 Feb 9;28(3):396–411.