Description
This track shows the BLAT alignments of transcripts assembled
by Trinity using the
genome-guided approach. RNA-Seq data from different D. ananassae samples were assembled
separately and then combined using the tr2aacds.pl program
in EvidentialGene. Only
the transcripts that have been classified by EvidentialGene as "okay" were used in the BLAT
search.
Accession Numbers for RNA-Seq Data
The RNA-Seq data used to construct the transcriptome were obtained from NCBI
using the following accession numbers:
Adult Females and Adult Males RNA-Seq Data
Embryos RNA-Seq Data
Adult Tissues and Body Parts RNA-Seq Data
Carcass, Female Ovaries, Male Testes RNA-Seq Data
References
Gilbert DG. Longest protein, longest
transcript or most expression, for accurate gene reconstruction of transcriptomes? bioRxiv
829184; doi: https://doi.org/10.1101/829184
Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R,
Zeng Q, Chen Z, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren BW, Nusbaum C,
Lindblad-Toh K, Friedman N, Regev
A. Full-length transcriptome assembly from
RNA-Seq data without a reference genome. Nat Biotechnol. 2011 May 15;29(7):644-52.
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