Description
This track shows the exon junctions extracted from spliced RNA-Seq reads of multiple tissues of D. melanogaster. The RNA-Seq reads were obtained from the
ENCODE data portal at UCSC on August 2019, and then mapped against
the D. melanogaster dm6 genome assembly using
HISAT2.
The splice junctions in each sample were identified by the
regtools junctions extract
subprogram. The splice junction predictions from the different samples are filtered
and merged together into a single set of predictions. The predictions are
color-coded based on the number of reads supporting the junction:
| Color | Number of reads |
|---|
| > 1000 |
| 500-999 |
| 100-499 |
| 50-99 |
| 10-49 |
| < 10 |
References
Brown JB, et al.
Diversity and dynamics of the Drosophila transcriptome. Nature. 2014 Aug 28;512(7515):393-9.
Kim D, Langmead B, Salzberg SL.
HISAT: a fast spliced aligner with low memory requirements.
Nat Methods. 2015 Apr;12(4):357-60.
|